WATT PoultryUSA - January 2014 - 30
30 ❙ WATT POULTRY USA ■ FOOD SAFETY cultural procedure for the isolation and identification of Salmonella was used for the neck skin and whole-carcass rinse methods. carcass by one sampling method it was not detected by the other method, indicating that both methods can produce false negative results and by the nature of the methods, this should be obvious. No significant difference observed between methods Both methods produced false negative results The whole-carcass rinse and neck skin procedures produced results that were not significantly different (P>0.05) for Salmonella isolation when the same carcass was sampled by both methods (Table 1). However, there was a significant difference in Salmonella prevalence for carcasses sampled pre- and post-chill, with pre-chill carcasses having a much higher prevalence than post-chill samples. In the U.S., processing plants use immersion chilling, and a variety of antimicrobials may be applied in the chilling process and in the plant. In the EU, air chilling is more common and antimicrobials are currently not in use. Overall, there was no significant difference observed between the two methods (whole-carcass rinse and neck skin). However, in some cases, when Salmonella was detected on a With whole-carcass rinse only 7.5 percent (30 mL) of the rinsate is analyzed and this contributes to false negative results. In a 2008 Australian study (King, et al., 2008) using the same sampling method and the same carcass, Salmonella prevalence ranged from 20 percent to 48 percent depending on the volume of rinsate analyzed. When 30 mL was tested, the detection rate was 20 percent, when 50 mL was analyzed it was 38 percent, and when approximately 400 mL was analyzed it was 48 percent positive detection. In addition to this, firmly attached Salmonella will not be recovered by the whole-carcass rinse. However, the neck skin method is equally insensitive because only 3 percent to 4 percent of the total carcass skin is used for analysis. The total surface and visceral cavity are not part of the sample. Salmonella prevalence in commercial broiler carcasses obtained prechill and post-chill and analyzed by two different sampling methods Differing methods yielded comparable results Sampling method Sampling location Neck skin Whole-carcass rinse Pre-chill 49/1801 66/180 Post-chill 12/177 5/177 Total 61/357 71/357 1 Even though some insensitivity does exist, the fact that results with these two methods are similar allows one to compare Salmonella prevalence on broiler carcass studies conducted in the U.S. and the EU and other parts of the world using either method. ◼ Number of positives/number sampled N. A. Cox; J. A. Cason; M. E. Berrang; C. Hofacre; D. E. Cosby; R. Biggs; S. Wagener; A. M. Lammerding; and M. P. Doyle The whole-carcass rinse and neck-skin excision procedures produced results that were not significantly different for Salmonella isolation. Our Angle Is On Quality & Performance ® SPACESP A -RAY R SRB40CR Poultry Brooder A nti-Corrosion C hampion Anti-Corrosion Champio Ignition Control Moisture resistant enclosure Against Corrosion From Humidity And Ammonia Our Best Brooder Is Now Even Bett t er! tt T he N ew, IImp mprroved oved Spa ce-Ray SR B4 0CR B rooder The New, ace-Ray RB 40 Brooder Available * Pilot or Direct Spark Ignition (DSI) Models Av * DSI Models Also Feature New Dual Fuel Regulator. Aluminum Canopy Large deep dish canopy to eff f iciently reflect heat to litt ff t er area tt where it is needed most Stainles ss Steel Emitter Assembly DSI Model Shown Stainless Steel Gas Line & Safety Pan This make kes it easy to conve vert between natural & pro ropane gas. * 60° emittttter angle aims the radiant heat down to floor level, directly at the birds where it's needed most * Quick Release Bracket Provides Fast Access Too Main Burner Orifice Stainless steel emitt t er assembly,, fully insulated tt and enclosed. Will not crumble or distort, and feature es a full 3-year limited warranty.. Stainless Steel Burner Corrosion resistant stainless steel burner assembly features simple disassembly for easy cleaning and maintenance Visit Us At Booth 2059 - A Hall SPA SP PACE--RAY ® Call or visit our website for specification brochure Division of Gas Fired Products, Inc. Sinnce 1949 To oll Free 1-800-849-7311 (704)372-3488 Email: info@spaceray..com www.spaceray..com © 2013, GFP Inc.
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