Poultry USA - August 2019 - 40

40 ❙ WATTPoultryUSA
SALMONELLA DETECTION
persistence of Salmonella than pullets that were fed with
a skip-a-day program. Emptying of the crop every other
day, allowing for an increase in crop pH and litter picking
may contribute to a higher prevalence and persistence of
Salmonella positive ceca.

■ Salmonella sampling in slaughter plants
During turkey and broiler processing, multiple sampling
methods including whole carcass rinse, excision (skin or
muscle), swab, neck skin and whole carcass enrichment
have been evaluated for the detection of Salmonella.
For turkey carcasses, methods that require sampling of
a larger surface area of the carcass (whole carcass swabbing
and whole carcass rinse) are more sensitive than one or two
site swab sampling. Following immersion chilling, whole
carcass enrichment was the most sensitive method, neck skin
less sensitive and whole carcass rinse the least sensitive.
Although sampling methods vary in sensitivity,
each method has a minimal level of detection. The
most sensitive method (whole carcass enrichment)
requires approximately eight to 10 cells present on
the carcass for a positive result following 24 hours of
enrichment incubation.

An example of a hand grab of litter including feces
Courtesy Dianna Bourassa

Therefore, any product sampled can never really be
declared Salmonella-free. The absence of a positive result
from a carcass or 325 grams of comminuted product does
not indicate that there is no Salmonella present. Use of
the phrase zero tolerance may lead consumers to believe
that there is no Salmonella on raw product and may give a
false sense of security.

Serogroup diversity is impacted
by sampling and analysis methods

An example of a pre-moistened sock used for litter
sampling. Courtesy Dianna Bourassa

When the detection of Salmonella serogroups was
analyzed following three different carcass sampling
methods - neck skin, whole carcass rinse and whole
carcass enrichment - the greatest number of serogroups
was found with whole carcass enrichment (five serogroups) followed by neck skins (two serogroups), followed
by whole carcass rinse (one serogroup). The greater serogroup detection may be due to incubation of the carcass or
skin within the rinse media.
Incubation in the media would have allowed for
Salmonella that was tightly attached to the carcass to
be detected while the whole carcass rinse sample would
only contain those Salmonella that were more easily
rinsed off during sampling.
Sample enrichment and the use of selective media
are commonly used when culturing for Salmonella. If
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Poultry USA - August 2019

Table of Contents for the Digital Edition of Poultry USA - August 2019

Poultry USA - August 2019
Contents
Get ready for plant-based protein at KFC, Chick-fil-A
The poultry and egg industries’ sustainability program
Potential gains from ASF outweigh rising grain prices
How to get a processing line speed waiver
Consumers invested in animal welfare, but knowledge is limited
Lack of reliable detection technology slows PAA regulation
How sample type, analysis impact Salmonella detection
Product Review
Market Place
Ad Index
Poultry USA - August 2019 - CT1
Poultry USA - August 2019 - CT2
Poultry USA - August 2019 - Poultry USA - August 2019
Poultry USA - August 2019 - Cover2
Poultry USA - August 2019 - Contents
Poultry USA - August 2019 - Potential gains from ASF outweigh rising grain prices
Poultry USA - August 2019 - 3
Poultry USA - August 2019 - 4
Poultry USA - August 2019 - 5
Poultry USA - August 2019 - 6
Poultry USA - August 2019 - 7
Poultry USA - August 2019 - 8
Poultry USA - August 2019 - 9
Poultry USA - August 2019 - 10
Poultry USA - August 2019 - 11
Poultry USA - August 2019 - 12
Poultry USA - August 2019 - 13
Poultry USA - August 2019 - How to get a processing line speed waiver
Poultry USA - August 2019 - 15
Poultry USA - August 2019 - 16
Poultry USA - August 2019 - 17
Poultry USA - August 2019 - 18
Poultry USA - August 2019 - 19
Poultry USA - August 2019 - Consumers invested in animal welfare, but knowledge is limited
Poultry USA - August 2019 - 21
Poultry USA - August 2019 - 22
Poultry USA - August 2019 - 23
Poultry USA - August 2019 - 24
Poultry USA - August 2019 - 25
Poultry USA - August 2019 - 26
Poultry USA - August 2019 - 27
Poultry USA - August 2019 - 28
Poultry USA - August 2019 - 29
Poultry USA - August 2019 - Lack of reliable detection technology slows PAA regulation
Poultry USA - August 2019 - 31
Poultry USA - August 2019 - 32
Poultry USA - August 2019 - 33
Poultry USA - August 2019 - 34
Poultry USA - August 2019 - 35
Poultry USA - August 2019 - 36
Poultry USA - August 2019 - 37
Poultry USA - August 2019 - How sample type, analysis impact Salmonella detection
Poultry USA - August 2019 - 39
Poultry USA - August 2019 - 40
Poultry USA - August 2019 - 41
Poultry USA - August 2019 - Product Review
Poultry USA - August 2019 - 43
Poultry USA - August 2019 - Ad Index
Poultry USA - August 2019 - Cover3
Poultry USA - August 2019 - Cover4
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